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活细胞成像,以评估在斜接主轴扰动后元相定时和细胞命运的动态
JoVE Journal
Biology
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JoVE Journal
Biology
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
Please note that all translations are automatically generated.
Click here for the English version.
活细胞成像,以评估在斜接主轴扰动后元相定时和细胞命运的动态
DOI:
10.3791/60255-v
•
07:14 min
•
September 20, 2019
•
Dayna L. Mercadante*
1
,
Elizabeth A. Crowley*
1
,
Amity L. Manning
1
Department of Biology and Biotechnology
,
Worcester Polytechnic Institute
Chapters
00:04
Title
00:36
Live Cell Imaging Preparation
02:06
Microscope Setup
03:55
Time-Lapse Image Analysis
05:09
Results: Representative Time-Lapse Imaging of Mitotic Fidelity
06:32
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出一个协议,以评估主轴形成和线粒体进展的动态。我们的延时成像应用使用户能够识别线粒体不同阶段的细胞,跟踪和识别线粒体缺陷,并在接触抗线粒药物时分析主轴动力学和线粒细胞命运。
Tags
Live Cell Imaging
Metaphase
Mitotic Spindle Perturbations
Phototoxicity
Trypsin
Cell Culture
Mitotic Drug
Epifluorescence Microscope
Environmental Chamber
Numerical Aperture
Fluorescence
Phase Contrast
Bright Field Imaging
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