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活细胞成像,以评估在斜接主轴扰动后元相定时和细胞命运的动态
JoVE Journal
Biology
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JoVE Journal Biology
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
DOI:

07:14 min

September 20, 2019

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Chapters

  • 00:04Title
  • 00:36Live Cell Imaging Preparation
  • 02:06Microscope Setup
  • 03:55Time-Lapse Image Analysis
  • 05:09Results: Representative Time-Lapse Imaging of Mitotic Fidelity
  • 06:32Conclusion

Summary

Automatic Translation

在这里,我们提出一个协议,以评估主轴形成和线粒体进展的动态。我们的延时成像应用使用户能够识别线粒体不同阶段的细胞,跟踪和识别线粒体缺陷,并在接触抗线粒药物时分析主轴动力学和线粒细胞命运。

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