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Developmental Biology

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Quantification of Self-renewal in Murine Mammosphere Cultures

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Quantification of Self-renewal in Murine Mammosphere Cultures

Quantification of Self-renewal in Murine Mammosphere Cultures

DOI:

10.3791/60256-v

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07:40 min

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November 26, 2019

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Thalia Vlachou, Xieraili Aobuli, Errico D’Elia, Angela Santoro, Maria Cristina Moroni, Pier Giuseppe Pelicci²

¹Department of Experimental Oncology, IEO,European Institute of Oncology IRCCS, ²Department of Oncology and Hemato-Oncology,University of Milan

Chapters

00:04Title
01:09Murine Mammary Gland Collection and Digestion
01:52Primary Murine Mammary Epithelial Cell (MEC) Isolation
03:15Serial Mammosphere Re-Plating
04:12Sphere Enumeration by Digital Image Analysis (DIA)
05:45Cumulative Growth Curve Calculation
06:38Results: Representative Sphere and Cell Growth Numbers and Curves
07:05Conclusion

Summary

Automatic Translation

Here, we describe the implementation and interpretation of the results of an in vitro mammosphere self-renewal quantitative assay.

Tags

Keywords: Mammosphere Culture Self-renewal Breast Stem Cells Quantification Cell Culture Cell Isolation Cell Counting Cell Lysis Ultra-low Adhesion Primary Mammosphere Formation

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