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使用标准实验室设备从小鼠测试中浓缩帕西滕苯细胞和精子
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
DOI:

10:22 min

September 17, 2019

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Chapters

  • 00:04Title
  • 00:58Animal Dissection and Preparation of Germ Cell Suspension
  • 04:50Separation of Germ Cells Through the Discontinuous BSA Gradient
  • 06:02Collection of Enriched Germ Cell Fractions
  • 06:52Analysis of Cell Fractions
  • 08:06Results: MDR Cell Enrichment
  • 09:29Conclusion

Summary

Automatic Translation

这里介绍的是一个协议,用于使用标准实验室设备,使用不连续的牛血清白蛋白密度梯度,丰富白细胞、圆形精子和从成年小鼠睾人身上拉长精子。

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