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Developmental Biology
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使用标准实验室设备从小鼠测试中浓缩帕西滕苯细胞和精子
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
Please note that all translations are automatically generated.
Click here for the English version.
使用标准实验室设备从小鼠测试中浓缩帕西滕苯细胞和精子
DOI:
10.3791/60271-v
•
10:22 min
•
September 17, 2019
•
Matteo Da Ros*
1
,
Tiina Lehtiniemi*
1
,
Opeyemi Olotu
,
Oliver Meikar
,
Noora Kotaja
1
Institute of Biomedicine
,
University of Turku
Chapters
00:04
Title
00:58
Animal Dissection and Preparation of Germ Cell Suspension
04:50
Separation of Germ Cells Through the Discontinuous BSA Gradient
06:02
Collection of Enriched Germ Cell Fractions
06:52
Analysis of Cell Fractions
08:06
Results: MDR Cell Enrichment
09:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一个协议,用于使用标准实验室设备,使用不连续的牛血清白蛋白密度梯度,丰富白细胞、圆形精子和从成年小鼠睾人身上拉长精子。
Tags
Pachytene Spermatocytes
Spermatids
Mouse Testes
MDR Method
Standard Laboratory Equipment
Spermatogenesis
Male Fertility
Paternal Epigenetic Inheritance
Testis Dissection
Collagenase
Trypsin
Cell Separation
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