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Chapters
- 00:05Title
- 00:50Tube and Cell Preparation
- 03:01Cell Sorting
- 04:50Results: Validation of the Purification Scheme by Epifluorescence Microscopy
- 05:46Conclusion
We describe a sorting strategy for mouse spermatids using flow cytometry. Spermatids are sorted into four highly pure populations, including round (spermiogenesis steps 1-9), early elongating (spermiogenesis steps 10-12), late elongating (spermiogenesis steps 13-14) and elongated spermatids (spermiogenesis steps 15-16). DNA staining, size and granulosity are used as selection parameters.
