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巨噬细胞报告器细胞分析,以检查聚合物表面的吸附蛋白层上的收费类似受体介导NF-kB/AP-1信号
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Bioengineering
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JoVE Journal
Bioengineering
A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
Please note that all translations are automatically generated.
Click here for the English version.
巨噬细胞报告器细胞分析,以检查聚合物表面的吸附蛋白层上的收费类似受体介导NF-kB/AP-1信号
DOI:
10.3791/60317-v
•
07:55 min
•
January 07, 2020
•
Laura A. McKiel
,
Kimberly A. Woodhouse
,
Lindsay E. Fitzpatrick
1
Department of Chemical Engineering
,
Queen’s University
Chapters
00:04
Title
00:34
Cell Culture Surface Coating with Poly(Methyl Methacrylate) (PMMA)
02:15
3T3 Cell Lysate Preparation
03:26
Adsorbed Protein Layer Effect on Toll-Like Receptor (TLR)-Mediated Nuclear Factor-kappa-B (NF-kappa-B) Macrophage Activity Assessment
06:01
Results: Representative Lysate TLR Ligand Adsorption to Different Polymer Surfaces
07:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议为研究人员提供了一种快速、间接的方法,用于测量小鼠巨噬细胞系中与TLR相关的NF-B/AP-1转录因子活性,以响应各种聚合物表面和吸附蛋白层,这些蛋白质层为生物材料植入微环境建模。
Tags
Macrophage Reporter Cell Assay
Toll-like Receptor
NF-kB
AP-1
Signaling
Adsorbed Protein Layers
Polymeric Surfaces
PMMA
Spin Coating
3T3 Cell Lysates
Cell Culture
Cell Dissociation
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