Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Bioengineering

This content is Open Access.

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
 

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Article DOI: 10.3791/54447
September 15th, 2016

Chapters

Summary September 15th, 2016

We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter