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Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

DOI:
10.3791/54447-v

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12:19 min

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September 15, 2016

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, , ,
1IBM Research – Zurich

Chapters

  • 00:05Title
  • 01:16Microfluidic Probe (MFP) Head and Platform Cleaning and Preparation
  • 06:13Creating the Hierarchical Hydrodynamic Flow Confinement (hHFC) and Patterning Cell Monolayers Using hHFC
  • 08:10Downstream Processing for Sampling and DNA Amplification
  • 09:46Results: Rapid Subtractive Patterning of Cell Monolayers Using a Hierachical HFC
  • 10:54Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.

Tags

Keywords: Microfluidic ProbeCell PatterningSpatiotemporal AnalysisCell-cell InteractionsRapid PatterningLive Cell LayersSubtractive PatterningHydrodynamic Flow ConfinementMotorized StagesFluid HandlingSyringe PumpsCapillaries

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