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Immunology and Infection
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Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

DOI:
10.3791/60421-v

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10:40 min

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December 21, 2019

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, ,
1National Center for Advancing Translational Sciences,National Institutes of Health, 2United States Naval Research Laboratory

Chapters

  • 00:05Title
  • 01:01Identification of SSHHPS in the Host Genome Using BLAST
  • 01:49Design and Preparation of Protease Substrates
  • 04:07Continuous and Discontinuous Enzyme Assays
  • 06:02Docking Substrate Peptides to the VEEV-nsP2 Cysteine Protease
  • 08:27Results: SSHHPS Analysis
  • 09:54Conclusion

Summary

Automatic Translation

Automatic Translation

We present a general protocol for identifying short stretches of homologous host-pathogen protein sequences (SSHHPS) embedded in the viral polyprotein. SSHHPS are recognized by viral proteases and direct the targeted destruction of specific host proteins by several Group IV viruses.

Tags

Keywords: Group IV Viral GenomesViral Protease Cleavage SitesHost Protein SequencesImmune ResponseProtease AssaysZika SSHHPS SequencesBrain And Eye DevelopmentProtein BLASTPHI-BLASTCFP-YFP SubstratesBacterial ExpressionProtein Purification

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