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研究肿瘤分泌性副体结扎对宏噬细胞活化的影响,使用可渗透膜支架共培养
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
DOI:

07:44 min

November 28, 2019

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Chapters

  • 00:04Title
  • 01:00Macrophage Culture
  • 01:32Co-culture of Tumor Cells with Macrophages
  • 02:56Treatment of Co-cultured Cells
  • 03:52Downstream Analysis of Co-cultured Cells
  • 04:38Results: The Effects of Tumor-secreted Paracrine Ligands on Macrophage Activation
  • 07:12Conclusion

Summary

Automatic Translation

在这里,我们提出了一种使用渗透膜支架的方法,以促进肿瘤细胞用于抑制免疫反应的非接触性对羟基苯甲酰二甲苯信号的研究。该系统有助于研究肿瘤分泌因子在抑制巨噬菌体活化中的作用。

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