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Cancer Research
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研究肿瘤分泌性副体结扎对宏噬细胞活化的影响,使用可渗透膜支架共培养
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
Please note that all translations are automatically generated.
Click here for the English version.
研究肿瘤分泌性副体结扎对宏噬细胞活化的影响,使用可渗透膜支架共培养
DOI:
10.3791/60453-v
•
07:44 min
•
November 28, 2019
•
Kelly Pittman
,
Shelton Earp
,
Eric Ubil
1
Lineberger Comprehensive Cancer Center
,
University of North Carolina at Chapel Hill
,
2
O’Neal Comprehensive Cancer Center
,
University of Alabama at Birmingham
Chapters
00:04
Title
01:00
Macrophage Culture
01:32
Co-culture of Tumor Cells with Macrophages
02:56
Treatment of Co-cultured Cells
03:52
Downstream Analysis of Co-cultured Cells
04:38
Results: The Effects of Tumor-secreted Paracrine Ligands on Macrophage Activation
07:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种使用渗透膜支架的方法,以促进肿瘤细胞用于抑制免疫反应的非接触性对羟基苯甲酰二甲苯信号的研究。该系统有助于研究肿瘤分泌因子在抑制巨噬菌体活化中的作用。
Tags
Tumor-secreted Paracrine Ligands
Macrophage Activation
Co-culture
Permeable Membrane
Immune Suppression
Paracrine Signaling
Cell-to-cell Contact
Molecular Biological Techniques
Peritoneal Macrophages
Tumor Cells
DMEM
Interferon Gamma
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