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人类粪便标本中弯曲杆菌Jejuni在运输介质中确定检测和生存极限的培养方法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
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人类粪便标本中弯曲杆菌Jejuni在运输介质中确定检测和生存极限的培养方法

DOI:
10.3791/60457-v

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08:23 min

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March 10, 2020

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, ,
1TECHLAB, Inc.

Chapters

  • 00:04Introduction
  • 01:04Campylobacter Enumeration in Contrived Human Fecal Specimens
  • 03:42Contrived Clinical Fecal Specimen Preparation and Enumeration
  • 05:21Determination of Campylobacter Viability in Transport Medium
  • 06:17Results: Representative Typical Numbers of Colonies on Plates of Spiked Fecal Samples
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

虽然弯曲杆菌的粪便培养不精确，但它仍然被认为是识别的黄金标准。介绍了确定人类粪便中C.jejuni运输介质检测和生存极限的方法，并与一种精度更高的新免疫测定进行了比较。

Tags

Campylobacter JejuniCultureDetectionSurvivalTransport MediaFecal SpecimensLimit Of DetectionAnaerobicMicroaerobicOptical DensityDilution SeriesPlate Counting

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