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Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

DOI:
10.3791/56373-v

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09:50 min

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September 29, 2017

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, , , , ,
1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine,University of Calgary, 2Dept. of Molecular Biology,University of Wyoming, 3Canadian Food Inspection Agency,Lethbridge Laboratories

Chapters

  • 00:05Title
  • 00:42Preparation of Cervid Feces Etracts
  • 01:48Concentration of PrP
  • 03:33Real-time Quaking-induced Conversion Assay
  • 07:28Results: Detection of CWD Prions in Fecal Material
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to describe a simple, fast and efficient prion amplification technique, the real-time quaking-induced conversion (RT-QuIC) method.

Tags

Keywords: CWD PrionsFecal MaterialReal-time Quaking-induced Conversion AssayCervidChronic Wasting DiseaseSensitive DetectionHigh ThroughputSodium Phosphotungstic Acid PrecipitationRT-QuIC AssayThioflavin T

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