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Immunology and Infection
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結核分子細菌負荷アッセイ(TB-MBLA)
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
Please note that all translations are automatically generated.
Click here for the English version.
結核分子細菌負荷アッセイ(TB-MBLA)
DOI:
10.3791/60460-v
•
10:41 min
•
April 30, 2020
•
Wilber Sabiiti
,
Bariki Mtafya
2
,
Daniela Alferes De Lima
,
Evelin Dombay
,
Vincent O. Baron
,
Khalide Azam
,
Katarina Oravcova
,
Derek J. Sloan
,
Stephen H. Gillespie
1
Division of Infection and Global Health, School of Medicine
,
University of St Andrews
,
2
National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre
,
3
Instituto Nacional de Saúde (INS), Ministério da Saúde
,
4
Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences
,
University of Glasgow
Chapters
00:05
Introduction
01:33
Sample Preparation and Heat Inactivation
02:30
RNA Extraction
06:28
Reverse Transcriptase qPCR
07:57
Results: Heat Inactivation of
M. tuberculosis
Bacilli
09:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
痰の熱不活化後に行われる結核分子細菌負荷検定試験について述べている。熱不活性化は痰サンプルを非感染にし、結核分子試験のための封じ込めレベル3の実験室の必要性を排除する。
Tags
Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
Tuberculosis
Disease Burden
Anti-tuberculosis Medication
Treatment Response
Quantitative Result
Nontuberculous Mycobacteria
Chronic Obstructive Pulmonary Disease
Bacille Calmette-Guerin (BCG)
Sputum Samples
RNA Extraction
Phenol Chloroform
Extraction Control
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