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Cancer Research

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JoVE Journal Cancer Research
Kultur, manipulation och orthotopic transplantation av mus blåsa norgan organoider
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Kultur, manipulation och orthotopic transplantation av mus blåsa norgan organoider

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Detta protokoll ger detaljerade experimentella steg för att upprätta en tredimensionell in vitro kultur av urinblåsan tumör organoider härrör från cancerframkallande-inducerad urinblåsan cancer. Kulturmetoder inklusive passaging, genteknik och orthotopic transplantation av tumör organoider beskrivs.

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Cancerforskning tumör organoider 3D-kultur urinblåsan tumör invasivurothelial carcinom lentiviral transduktion ortotopic transplantation
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