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Adskillelse af cellekonvolutten for Gram-negative bakterier i indre og ydre membranfraktioner med tekniske justeringer for Acinetobacter baumannii
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Chapters
- 00:04Introduction
- 00:55Preparation of Bacteria for Membrane Extraction
- 02:18Dissociation of the Outer Membrane and Plasmolysis
- 03:56Pressurized Homogenization and Lysis
- 04:35Total Membrane Fractionation
- 05:34Separating the Dual Membranes
- 08:00Results: Purity and Quality of Inner and Outer Membrane Fractions
- 09:36Conclusion
Gram-negative bakterier producerer to rumligt adskilte membraner. Den ydre membran er opdelt fra den indre membran af en periplasm og et peptidoglycan lag. Evnen til at isolere de dobbelte tolag af disse mikrober har været afgørende for at forstå deres fysiologi og patogenese.
Tags
Cell EnvelopeGram-negative BacteriaInner MembraneOuter MembraneLipidsProteinsSugarsAntibiotic KillingAntimicrobial ResistanceDisease PathogenesisAcinetobacter BaumanniiCell FractionationDetergent-freeSemi-native EnvironmentOptical DensityCentrifugationLysozymeEDTAProtease InhibitorMagnesium ChlorideNuclease
