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人体神经细胞在体外热的化学和分子分析的生物粒子微阵列
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Bioengineering
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JoVE Journal Bioengineering
Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
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人体神经细胞在体外热的化学和分子分析的生物粒子微阵列

DOI:
10.3791/60544-v

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11:21 min

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February 16, 2020

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,
1William G. Lowrie Department of Chemical and Biomolecular Engineering,The Ohio State University, 2Comprehensive Cancer Center,The Ohio State University

Chapters

  • 00:04Introduction
  • 00:37Microfabrication of Bioparticle Microarray
  • 06:14Sample Preparation and Assay
  • 07:32Supernatant Collection
  • 08:40Results: Neutrophil Swarming Around Bioparticle Clusters
  • 10:45Conclusion

Summary

Automatic Translation

Automatic Translation

该协议生成生物粒子微阵列，提供空间控制的嗜中性粒细胞群。它便于接触嗜中性粒细胞在迁移过程中释放的介质，并允许定量成像分析。

Tags

Bioparticle MicroarraysNeutrophil SwarmingIn Vitro AnalysisCytokinesLipid MediatorsMigrationMonocytesT CellsCationic PolyelectrolitePhotolithographyPolydimethylsiloxanePDMSBiopsy Punch

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