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Developmental Biology
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心肌机械变形(DIAMOND)的位移分析揭示了胚胎斑马鱼心脏功能的分段异质性
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Developmental Biology
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JoVE Journal
Developmental Biology
Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
心肌机械变形(DIAMOND)的位移分析揭示了胚胎斑马鱼心脏功能的分段异质性
DOI:
10.3791/60547-v
•
09:15 min
•
February 06, 2020
•
Junjie Chen
,
René R. Sevag Packard
2,3
1
Division of Cardiology, Department of Medicine
,
David Geffen School of Medicine at University of California, Los Angeles
,
2
Ronald Reagan UCLA Medical Center
,
3
Veterans Affairs West Los Angeles Medical Center
Chapters
00:04
Introduction
01:13
Reconstruction of the 3D Systolic and Diastolic Heart and Segmentation of the Ventricle
02:41
Creation of Rectangular Parallelepipeds for Image Registration and Resampling of Systolic and Diastolic 3D Hearts Along the Short Axis Plane
05:01
Division of the Resampled Heart
06:20
Registration of Systolic and Diastolic Image Matrices and Output of Displacement Vectors
07:01
Results: Segmental Heterogeneity in Cardiac Function and Susceptibility to Chemotherapy-induced Injury
08:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议的目的是详细说明一种在生理和病理条件下评估胚胎斑马鱼分段心脏功能的新方法。
Tags
– Displacement Analysis Of Myocardial Mechanical Deformation (DIAMOND)- Segmental Cardiac Function- Zebrafish Embryos- Quantitative Assessment- Four-dimensional- Cardiac Mechanics- Doxorubicin-induced Cardiac Toxicity- High-throughput In Vivo Screening- Mammalian Heart- Muscle Fiber Orientation- Rotational And Twisting Motion- Anatomical Structure- Ventricle Segmentation- 3D TIF Files- Image Analysis Software- Prepimage 1.m
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