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Developmental Biology
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心筋機械変形(DIAMOND)の変位解析は、胚性ゼブラフィッシュにおける心機能のセグメント的不均一性を明らかにする
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Developmental Biology
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JoVE Journal
Developmental Biology
Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
心筋機械変形(DIAMOND)の変位解析は、胚性ゼブラフィッシュにおける心機能のセグメント的不均一性を明らかにする
DOI:
10.3791/60547-v
•
09:15 min
•
February 06, 2020
•
Junjie Chen
,
René R. Sevag Packard
2,3
1
Division of Cardiology, Department of Medicine
,
David Geffen School of Medicine at University of California, Los Angeles
,
2
Ronald Reagan UCLA Medical Center
,
3
Veterans Affairs West Los Angeles Medical Center
Chapters
00:04
Introduction
01:13
Reconstruction of the 3D Systolic and Diastolic Heart and Segmentation of the Ventricle
02:41
Creation of Rectangular Parallelepipeds for Image Registration and Resampling of Systolic and Diastolic 3D Hearts Along the Short Axis Plane
05:01
Division of the Resampled Heart
06:20
Registration of Systolic and Diastolic Image Matrices and Output of Displacement Vectors
07:01
Results: Segmental Heterogeneity in Cardiac Function and Susceptibility to Chemotherapy-induced Injury
08:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの目標は、生理学的および病理学的条件の下で胚性ゼブラフィッシュにおけるセグメント性心機能の評価のための新しい方法を詳述することです。
Tags
– Displacement Analysis Of Myocardial Mechanical Deformation (DIAMOND)- Segmental Cardiac Function- Zebrafish Embryos- Quantitative Assessment- Four-dimensional- Cardiac Mechanics- Doxorubicin-induced Cardiac Toxicity- High-throughput In Vivo Screening- Mammalian Heart- Muscle Fiber Orientation- Rotational And Twisting Motion- Anatomical Structure- Ventricle Segmentation- 3D TIF Files- Image Analysis Software- Prepimage 1.m
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