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Förskjutning analys av hjärtinfarkt mekanisk deformation (DIAMOND) avslöjar segmental heterogenitet av hjärtfunktion i embryonalzebrafisk
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish
DOI:

09:15 min

February 06, 2020

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Chapters

  • 00:04Introduction
  • 01:13Reconstruction of the 3D Systolic and Diastolic Heart and Segmentation of the Ventricle
  • 02:41Creation of Rectangular Parallelepipeds for Image Registration and Resampling of Systolic and Diastolic 3D Hearts Along the Short Axis Plane
  • 05:01Division of the Resampled Heart
  • 06:20Registration of Systolic and Diastolic Image Matrices and Output of Displacement Vectors
  • 07:01Results: Segmental Heterogeneity in Cardiac Function and Susceptibility to Chemotherapy-induced Injury
  • 08:45Conclusion

Summary

Automatic Translation

Målet med detta protokoll är att detalj en ny metod för bedömning av segmental hjärt funktion i embryonala zebrafisk under både fysiologiska och patologiska förhållanden.

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