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Developmental Biology
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利用人类胚胎干细胞的单细胞培养进行高效神经分化
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Developmental Biology
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JoVE Journal
Developmental Biology
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
利用人类胚胎干细胞的单细胞培养进行高效神经分化
DOI:
10.3791/60571-v
•
11:17 min
•
January 18, 2020
•
Kilsoo Jeon
,
Kyeyoon Park
,
Anton M. Jetten
1
Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences
,
National Institutes of Health
,
2
NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke
,
National Institutes of Health
Chapters
00:05
Title
01:05
Adaptation of Colony Type hESCs to Single-cell hESC Culture
04:01
Embryoid Body Formation and Differentiation
05:44
NPC Expansion and Cryopreservation
06:41
Differentiation of NPCs into Dopaminergic Neurons and Astrocytes
08:54
Results: In vitro Differentiation of Adapted Single-cell type hESCs
10:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是人类胚胎干细胞单细胞培养及其随后分化为神经祖细胞的一个方案。该协议简单、可靠、可扩展,适用于药物筛选和再生医学应用。
Tags
Human Embryonic Stem Cells
Neural Progenitor Cells
Single-cell Culture
Efficient Differentiation
Scalable Culture System
Multi-step Differentiation
Neural Lineages
Basement Membrane Matrix
Cell Detachment
MTeSR-1 Medium
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