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A High Output Method to Isolate Cerebral Pericytes from Mouse
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A High Output Method to Isolate Cerebral Pericytes from Mouse

A High Output Method to Isolate Cerebral Pericytes from Mouse

DOI:
10.3791/60588-v

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06:49 min

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January 14, 2020

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, , , , ,
1Laboratory of the Blood Brain Barrier,University of Artois, 2Department of Neurology, Universitätsmedizin Mannheim,Heidelberg University

Chapters

  • 00:04Title
  • 00:44Brain Tissue Recovery and Meninges Removal
  • 01:31Homogenization
  • 02:18Vascular Fraction Isolation
  • 04:32Cerebral Pericyte Proliferation
  • 05:25Results: Representative Cell Morphology, Marker Expression, and Culture Purity
  • 06:25Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.

Tags

Keywords: Cerebral PericytesPericyte IsolationMouse BrainIn Vitro StudiesCell CultureCell YieldHigh PurityHomogenizationVascular FractionBovine Serum Albumin DextranCell SuspensionFiltration

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