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Multiplexed enkelt celle mRNA sekvensering analyse af mus embryonale celler
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
DOI:

08:30 min

January 07, 2020

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Chapters

  • 00:04Title
  • 00:41Lipid-based Barcoding
  • 01:36Droplet Generation and mRNA Reverse Transcription
  • 03:43Post Single Cell Reverse Transcription Cleanup
  • 05:18cDNA Amplification
  • 06:42Results: Representative Multiplexed Single Cell mRNA Sequencing Analysis
  • 07:50Conclusion

Summary

Automatic Translation

Her præsenterede vi en multiplexed enkelt celle mRNA sekvensering metode til profil genekspression i mus embryonale væv. Den droplet-baserede enkelt celle mRNA sekventering (scrna-SEQ) metode i kombination med multiplexing strategier kan profilere enkeltceller fra flere prøver samtidigt, hvilket reducerer reagens omkostningerne betydeligt og minimerer eksperimentelle batch effekter.

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