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原発性歯槽上皮細胞におけるmRNA転写物の安定性の研究のための効率的な転写制御プラスミド発現システム
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JoVE Journal
Genetics
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
原発性歯槽上皮細胞におけるmRNA転写物の安定性の研究のための効率的な転写制御プラスミド発現システム
DOI:
10.3791/60654-v
•
10:49 min
•
March 06, 2020
•
Francis Migneault
2,3
,
Frédéric Gagnon
3
,
Emmanuelle Brochiero
3
,
Yves Berthiaume
3
,
André Dagenais
3
1
Centre de Recherche du Centre hospitalier de l’Université de Montréal (CRCHUM)
,
2
Institut de Recherches Cliniques de Montréal (IRCM)
,
3
Département de médecine, Faculté de médecine
,
Université de Montréal
Chapters
00:05
Introduction
00:43
Response Plasmid Design and Generation
02:10
Response Plasmid Transfection
05:25
Transcription Inhibition
06:27
mRNA Stability Analysis
08:22
Results: Representative mRNA Transcript Stability Evaluation
10:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、ピペットエレクトロポレーション技術に結合した誘導発現系を用いて、一次肺胞上皮細胞における転写物の転写後変調を研究するために使用できるツールを提示する。
Tags
Transcriptional Control
Plasmid Expression System
MRNA Stability
Primary Alveolar Epithelial Cells
Transient Transfection
Response Plasmid
Gene Of Interest
V5 Epitope
3′ UTR
Restriction Analysis
Sequencing
Primary Rat Lung Type II Alveolar Epithelial Cells
Cell Culture
Transfection
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