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Journal / Biochemistry / PeptiQuick, en ett-trinns innlemmelse av membran…
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Biochemistry
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JoVE Journal Biochemistry
PeptiQuick, en ett-trinns innlemmelse av membran proteiner i Biotinylated Peptidiscs for strømlinjeformet protein bindende analyser
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
Journal (Biochemistry)
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Journal (Biochemistry)
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
 
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PeptiQuick, en ett-trinns innlemmelse av membran proteiner i Biotinylated Peptidiscs for strømlinjeformet protein bindende analyser

Article DOI: 10.3791/60661-v • 15:04 min • November 2nd, 2019
November 2nd, 2019 •
1, 2, 1
1Department of Biochemistry, University of British Columbia, 2School of Biochemistry, University of Bristol

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Vi presenterer en metode som kombinerer membran protein rensing og rekonstituering til peptidiscs i ett enkelt kromatografiske trinn. Biotinylated stillaser brukes for direkte overflatefeste og måling av protein-ligand interaksjoner via biolayer laboratoriefasiliteter.

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Biokjemi membran mimetics nanodiscs membran proteiner reseptorer kanaler transportører Biosensor lipider colicin biotin-streptavidin protein-protein interaksjoner
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