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Biochemistry

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JoVE Journal Biochemistry
Trinvis doseringprotokol for øget overførselstid i label-free impedansbaserede GPCR-analyser
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Trinvis doseringprotokol for øget overførselstid i label-free impedansbaserede GPCR-analyser

Article DOI: 10.3791/60686-v • 06:13 min • February 21st, 2020
February 21st, 2020 •
1, 1, 1, 1, 2, 2, 3, 3, 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

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Denne protokol viser real-time registrering af fuld dosis-respons relationer for agonist-induceret GPCR aktivering fra en enkelt celle lag dyrket på en enkelt mikroelektrode ved hjælp af label-fri impedans målinger. Den nye doseringsordning øger gennemløb betydeligt uden tab i tidsløsning.

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Biokemi G-protein koblet receptor (GPCR) impedans etiketfri cellebaseret analyse koncentrationsresponsforhold agonist antagonist histamin
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