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Journal / Biochemistry / Trinnvis Doseringsprotokoll for økt…
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Biochemistry

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JoVE Journal Biochemistry
Trinnvis Doseringsprotokoll for økt gjennomstrømming i etikettfrie impedansbaserte GPCR-analyser
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Trinnvis Doseringsprotokoll for økt gjennomstrømming i etikettfrie impedansbaserte GPCR-analyser

Article DOI: 10.3791/60686-v • 06:13 min • February 21st, 2020
February 21st, 2020 •
1, 1, 1, 1, 2, 2, 3, 3, 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

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Denne protokollen demonstrerer sanntidsregistrering av full doseresponsrelasjoner for agonist-indusert GPCR-aktivering fra et enkeltcellelag dyrket på en enkelt mikroelektrode ved hjelp av etikettfrie impedansmålinger. Den nye doseringsordningen øker gjennomstrømningen betydelig uten tap i tidsoppløsning.

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Biokjemi Utgave 156 G-protein kombinert reseptor (GPCR) impedans etikettfri cellebasert analyse konsentrasjonsrespons forhold agonist antagonist histamin
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