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Kombineret brug af hale vene metastase assays og Real-Time in vivo billeddannelse at kvantificere brystkræft metastatisk kolonisering og byrde i lungerne
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
DOI:

10:32 min

December 19, 2019

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Chapters

  • 00:04Title
  • 00:32Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein
  • 01:59Tail Vein Injection of Labeled Cancer Cells
  • 04:17Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device
  • 06:18Quantification of the Number and Size of Metastases and Analysis of the Imaging Data
  • 08:01Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice
  • 09:36Conclusion

Summary

Automatic Translation

Den beskrevne fremgangsmåde kombinerer eksperimentelle hale vene metastase assays med in vivo levende dyr billeddannelse at tillade real-time overvågning af brystkræft metastase dannelse og vækst i tillæg til kvantificering af metastase antal og størrelse i lungerne.

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