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结合使用尾静脉转移分析与实时 Vivo 成像,以量化乳腺癌转移殖民化和肺负担
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Cancer Research
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JoVE Journal
Cancer Research
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
Please note that all translations are automatically generated.
Click here for the English version.
结合使用尾静脉转移分析与实时 Vivo 成像,以量化乳腺癌转移殖民化和肺负担
DOI:
10.3791/60687-v
•
10:32 min
•
December 19, 2019
•
Janine S. A. Warren
,
Paul J. Feustel
,
John M. Lamar
1
Department of Molecular and Cellular Physiology
,
Albany Medical College
,
2
Department of Neuroscience and Experimental Therapeutics
,
Albany Medical College
Chapters
00:04
Title
00:32
Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein
01:59
Tail Vein Injection of Labeled Cancer Cells
04:17
Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device
06:18
Quantification of the Number and Size of Metastases and Analysis of the Imaging Data
08:01
Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice
09:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
所述方法将实验性尾静脉转移测定与活体动物成像相结合,除了对肺部转移数和大小进行定量外,还能够实时监测乳腺癌转移的形成和生长。
Tags
Tail Vein Metastasis Assay
Real-time In Vivo Imaging
Breast Cancer
Metastatic Colonization
Metastasis Burden
Lung
T1 Cells
Luciferase
Fluorescent Protein
Viral Transduction
Cell Culture
Cell Counting
Cell Injection
Animal Model
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