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1Department of Anesthesiology and Critical Care, Soroka Medical Center,Ben-Gurion University of the Negev, 2Department of Anesthesiology,Yale University School of Medicine, 3Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, 4Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev
Chapters
- 00:03Introduction
- 00:23Preparing Rats for the Experimental Procedure
- 01:15Determination of Serum GOT, GPT and Total Bilirubin Baseline Level
- 01:44The Procedure for Induction of Acute Liver Injury in Rats
- 03:03Determination of Serum GOT, GPT and Total Bilirubin Level after 24 Hours
- 03:23Liver Collection for Histological Examination
- 04:03Histological Examination on Rats at Time 24 Hours
- 04:49Results
- 05:38Conclusion
Este protocolo descreve um método comum e viável de induzir lesão hepática aguda (ALI) através da exposição ao ClC4 através de uma sonda orogástrica. A exposição ao CCl4 induz ali através da formação de espécies reativas de oxigênio durante sua biotransformação no fígado. Este método é utilizado para analisar a fisiopatologia da ALI e examinar diferentes estratégias hepatoprotetoras.
