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JoVE Journal
Biochemistry

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KRAS4b ricombinante completamente elaborato
 
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KRAS4b ricombinante completamente elaborato: isolamento e caratterizzazione delle proteine di Farnesylated e Metylated

Article DOI: 10.3791/60703
January 16th, 2020

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Summary January 16th, 2020

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La prenilazione è una modifica importante sulle proteine che legano la membrana periferica. Le cellule degli insetti possono essere manipolate per produrre farnesylated e carboxymethylated KRAS4b in quantità che consentono misurazioni biofisiche delle interazioni proteina-proteina e proteina-lipidi

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