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JoVE Journal
Immunology and Infection

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TurboID-baseret nærhed mærkning for I Planta Identifikation af Protein-Protein Interaction Networks
 

TurboID-baseret nærhed mærkning for I Planta Identifikation af Protein-Protein Interaction Networks

Article doi: 10.3791/60728
May 17th, 2020

Summary May 17th, 2020

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Beskrevet her er en nærhed mærkning metode til identifikation af interaktion partnere tir domæne NLR immunreceptoren i Nicotiana benthamiana bladvæv. Der findes også en detaljeret protokol til identifikation af interaktioner mellem andre proteiner af interesse ved hjælp af denne teknik i Nicotiana og andre plantearter.

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