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使用全载免疫作用化学和三维重建对咽弓动脉的可视化与分析
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Developmental Biology
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JoVE Journal
Developmental Biology
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
Please note that all translations are automatically generated.
Click here for the English version.
使用全载免疫作用化学和三维重建对咽弓动脉的可视化与分析
DOI:
10.3791/60797-v
•
10:02 min
•
March 31, 2020
•
AnnJosette Ramirez
2
,
Sophie Astrof
1
Department of Cell Biology and Molecular Medicine, New Jersey Medical School
,
Rutgers Biomedical and Health Sciences
,
2
Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School
,
Rutgers Biomedical and Health Sciences
Chapters
00:04
Introduction
00:55
Embryo Staining and Embedding
03:03
Methanol Dehydration and Tissue Clearing
04:04
Embryo Mounting
04:40
Third Pharyngeal Arch (PA) Surfacing
05:57
Surfaced Structure Masking
07:01
Endothelial Cell Quantification
08:19
Results: Representative PA and PAA Surfacing and EC Quantification
09:28
Conclusion
Summary
Automatic Translation
English (Original)
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在这里,我们描述了一种协议,使用全孔免疫荧光、组织清除、共聚焦显微镜和3D重建来可视化和分析小鼠胚胎的咽弓动脉3、4和6。
Tags
Visualization
Analysis
Pharyngeal Arch Arteries
Whole-mount Immunohistochemistry
3D Reconstruction
Endothelial Cell Quantification
Aortic Arch Arteries
Congenital Heart Disease
Vessel Formation
Remodeling
Light-sheet Microscopy
Mouse Embryo
Fixation
Permeabilization
Blocking Buffer
Primary Antibody
Secondary Antibody
Plastic Paraffin Molds
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