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使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
DOI:

08:02 min

March 12, 2020

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Chapters

  • 00:05Introduction
  • 00:49Colon Sample Preparation
  • 03:12Immunohistochemistry
  • 06:07Results: Estrogen Receptor Localization in the TNBS-induced Crohn s Disease
  • 07:20Conclusion

Summary

Automatic Translation

该协议提出了完整的经验证的克罗恩病TNBS诱导的鼠模型,以及利用嵌入在石蜡中的正式固定结肠部分的免疫荧光,通过免疫组织化学对雌激素受体进行可视化的方法。

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