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Journal
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Biochemistry
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使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化
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Biochemistry
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JoVE Journal
Biochemistry
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
Please note that all translations are automatically generated.
Click here for the English version.
使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化
DOI:
10.3791/60813-v
•
08:02 min
•
March 12, 2020
•
Damian Jacenik
,
Marta Zielińska
,
Sylwia Michlewska
,
Jakub Fichna
,
Wanda M. Krajewska
1
Department of Cytobiochemistry, Faculty of Biology and Environmental Protection
,
University of Lodz
,
2
Department of Biochemistry, Faculty of Medicine
,
Medical University of Lodz
,
3
Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection
,
University of Lodz
Chapters
00:05
Introduction
00:49
Colon Sample Preparation
03:12
Immunohistochemistry
06:07
Results: Estrogen Receptor Localization in the TNBS-induced Crohn s Disease
07:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提出了完整的经验证的克罗恩病TNBS诱导的鼠模型,以及利用嵌入在石蜡中的正式固定结肠部分的免疫荧光,通过免疫组织化学对雌激素受体进行可视化的方法。
Tags
Estrogen Receptor
Immunofluorescence
TNBS-induced Crohn’s Disease
Mouse Colon
Paraffin Embedding
Microtome Sectioning
Deparaffinization
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