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Journal / Bioengineering / Förbereda proteinproducerande syntetiska celler…
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JoVE Journal Bioengineering
Förbereda proteinproducerande syntetiska celler med cellfria bakterieextrakt, liposomer och emulsion överföring
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Förbereda proteinproducerande syntetiska celler med cellfria bakterieextrakt, liposomer och emulsion överföring

Article DOI: 10.3791/60829-v • 09:37 min • April 27th, 2020
April 27th, 2020 •
*1,2, *1,2, *1,3, 1, 1,3, 1, 1, 1
1Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies, Department of Chemical Engineering, Technion - Israel Institute of Technology, 2The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology, Technion - Israel Institute of Technology, 3The Interdisciplinary Program for Biotechnology, Technion - Israel Institute of Technology
* These authors contributed equally

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Detta protokoll beskriver metod, material, utrustning och steg för nedifrån och upp-beredning av RNA och proteinproducerande syntetiska celler. Det inre vattenrummet i de syntetiska cellerna innehöll S30 bakteriella lysate inkapslade i en lipid bilayer (dvs. stabila liposomer), med hjälp av en vatten-i-olja emulsion överföringsmetod.

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Bioengineering Syntetiska celler cellfri emulsion bakteriell lysate konstgjorda celler läkemedelstillförsel terapeutiska proteiner GUVs liposom proteocell protocell proteoliposom biologiska riktad evolution syntetisk biologi
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