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Journal / Bioengineering / 使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞
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使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞

Article DOI: 10.3791/60829-v • 09:37 min • April 27th, 2020
April 27th, 2020 •
*1,2, *1,2, *1,3, 1, 1,3, 1, 1, 1
1Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies, Department of Chemical Engineering, Technion - Israel Institute of Technology, 2The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology, Technion - Israel Institute of Technology, 3The Interdisciplinary Program for Biotechnology, Technion - Israel Institute of Technology
* These authors contributed equally

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Summary
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该协议描述了用于自下而上制备RNA和产生合成细胞的蛋白质的方法、材料、设备和步骤。合成细胞的内水腔含有S30细菌裂解，封装在脂质双层层（即稳定脂质体）内，使用油水乳液转移方法。

Tags

生物工程， 问题 158， 合成细胞， 细胞自由， 乳液， 细菌裂解， 人工细胞， 药物输送， 治疗蛋白， GUV， 脂质体， 蛋白细胞， 原细胞， 蛋白质体， 生物制剂， 定向进化， 合成生物学
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