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Biochemistry

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JoVE Journal Biochemistry
Ungezielte Flüssigchromatographie-Massenspektrometrie-basierte Metabolomik-Analyse von Weizengetreide
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Ungezielte Flüssigchromatographie-Massenspektrometrie-basierte Metabolomik-Analyse von Weizengetreide

Article DOI: 10.3791/60851-v • 07:10 min • March 13th, 2020
March 13th, 2020 •
1,2,3, 1,4, 5,6, 1
1Research and Innovation, Murdoch University, 2Centre for Digital Agriculture, Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science, Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute, Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre, Murdoch University

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Eine Methode zur ungezielten Analyse von Weizenkornmetaboliten und Lipiden wird vorgestellt. Das Protokoll umfasst eine Acetonit-Metabolit-Extraktionsmethode und eine umgekehrte Phase-Flüssigchromatographie-Massenspektrometrie-Methodik mit Erfassung in positiven und negativen Elektrospray-Ionisationsmodi.

Tags

Biochemie Ausgabe 157 Weizen Weizen Weizensorte Weizengetreide Landwirtschaft Metabolomik ungezielte Metabolomik Flüssigchromatographie Massenspektrometrie
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