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Journal
/
Neuroscience
/
在
果蝇黑色无gaster
的Axon死亡期间,对Axon及其突触的形态和功能评估
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal
Neuroscience
Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in
Drosophila melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
在
果蝇黑色无gaster
的Axon死亡期间,对Axon及其突触的形态和功能评估
DOI:
10.3791/60865-v
•
10:29 min
•
March 16, 2020
•
Maria Paglione*
1
,
Arnau Llobet Rosell*
1
,
Jean-Yves Chatton
,
Lukas J. Neukomm
1
Department of Fundamental Neurosciences
,
University of Lausanne
Chapters
00:05
Introduction
01:28
Observation of Axon Morphology During Axon Death in the PNS (Peripheral Nervous System)
03:22
Observation of Axon and Synapse Morphology During Axon Death in the CNS (Central Nervous System)
05:53
Grooming Induced by Optogenetics as a Readout for Axon and Synapse Function
07:56
Results: Axon Death of GFP-Labeled Sensory Neuron Axons and Axonal/Synaptic Function After Axotomy
09:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提供协议,在
果蝇黑色素气
仪中执行三个简单的伤害诱发的斧子退化(斧子死亡)测定,以评估被切断的斧子及其突触的形态和功能保存。
Tags
Drosophila Melanogaster
Axon Degeneration
Axon Morphology
Synapse Function
Partial Wing Injury
Antennal Ablation
Optogenetics
Neuronal Maintenance Factors
Axonal Transport
Axonal Organelles
Spinning Disk Microscopy
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