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ショウジョウバエメラノガスター
における軸索死における軸索とそのシナプスの形態学的および機能的評価
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in
Drosophila melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
ショウジョウバエメラノガスター
における軸索死における軸索とそのシナプスの形態学的および機能的評価
DOI:
10.3791/60865-v
•
10:29 min
•
March 16, 2020
•
Maria Paglione*
1
,
Arnau Llobet Rosell*
1
,
Jean-Yves Chatton
,
Lukas J. Neukomm
1
Department of Fundamental Neurosciences
,
University of Lausanne
Chapters
00:05
Introduction
01:28
Observation of Axon Morphology During Axon Death in the PNS (Peripheral Nervous System)
03:22
Observation of Axon and Synapse Morphology During Axon Death in the CNS (Central Nervous System)
05:53
Grooming Induced by Optogenetics as a Readout for Axon and Synapse Function
07:56
Results: Axon Death of GFP-Labeled Sensory Neuron Axons and Axonal/Synaptic Function After Axotomy
09:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、ショ
ウジョウバエメラノガスター
で3つの単純な傷害誘発軸索変性(軸索死)アッセイを行い、切断された軸索とそのシナプスの形態学的および機能的保存を評価するプロトコルを提供する。
Tags
Drosophila Melanogaster
Axon Degeneration
Axon Morphology
Synapse Function
Partial Wing Injury
Antennal Ablation
Optogenetics
Neuronal Maintenance Factors
Axonal Transport
Axonal Organelles
Spinning Disk Microscopy
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