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Kombineret infusion og stimulering med hurtigscanning cyklisk voltammetri (CIS-FSCV) til vurdering af ventral tegmental område receptor regulering af Phasic dopamin
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JoVE Journal Neuroscience
Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Kombineret infusion og stimulering med hurtigscanning cyklisk voltammetri (CIS-FSCV) til vurdering af ventral tegmental område receptor regulering af Phasic dopamin

DOI:
10.3791/60886-v

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06:40 min

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April 23, 2020

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, , ,
1Department of Psychology,Elizabethtown College, 2Department of Psychiatry,Yale University, 3Department of Cellular and Molecular Physiology,Yale University

Chapters

  • 00:04Introduction
  • 00:56Electrode Implantation
  • 03:12Carbon Fiber and Stimulating Electrode/Guide Cannula Location Optimization
  • 04:26Combination Infusion and Stimulation Fast-Scan Cyclic Voltammetry (FSCV) Recording
  • 05:18Results: Representative Analyses of Stimulation-Evoked Phasic Dopamine Release
  • 06:03Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at direkte manipulere ventral tegmental område receptorer til at studere deres bidrag til subsekund dopamin frigivelse.

Tags

Ventral Tegmental AreaPhasic Dopamine ReleaseNucleus AccumbensReceptor FunctionCarbon Fiber MicroelectrodeBipolar StimulatorReference ElectrodeSterilizedLambdaBregmaScrewDuraBipolar Stimulating Electrode

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