JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
内
塞里亚淋病
的金属有限生长,用于金属反应基因特征和从宿主配体中获取金属
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Metal-Limited Growth of
Neisseria gonorrhoeae
for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
Please note that all translations are automatically generated.
Click here for the English version.
内
塞里亚淋病
的金属有限生长,用于金属反应基因特征和从宿主配体中获取金属
DOI:
10.3791/60903-v
•
07:26 min
•
March 04, 2020
•
Stavros Maurakis
,
Cynthia Nau Cornelissen
1
Institute for Biomedical Sciences
,
Georgia State University
Chapters
00:05
Introduction
00:31
Metal Limited
Neisseria gonorrhoeae
Growth
01:50
Metal Loading
02:36
Metal-Limited Growth Assay
03:50
Outer Membrane Metal Transporter Ligand Binding Detection
05:38
Results: Representative Differential Metal Responsive Protein Production, Growth-Restriction, and Binding
06:50
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们在这里描述了一种在金属受限液体介质中生长
淋病
的方法,以促进金属吸走基因的表达。我们还概述了下游实验,以表征在这些条件下生长的淋球菌的表型。这些方法可以适应其他细菌中金属反应基因的表征。
Tags
Metal-limited Growth
Neisseria Gonorrhoeae
Metal-responsive Genes
Metal Acquisition
Host Ligands
Nutritional Immunity
Metal Restricted Conditions
Bacterial Growth
CDM
Klett Colorimeter
Metal Loaded Proteins
Transferrin
Dialysis
96 Well Microplate
Optical Density
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
胞内相互作用的可视化和量化
脑膜炎奈瑟菌
和人类α-辅肌动蛋白共聚焦成像
解剖宿主 - 病毒相互作用模型疱疹病毒裂解性复制
利用RNA干扰调查的先天免疫反应的小鼠巨噬细胞
通过流式细胞仪大鼠肾脏巨噬细胞的表型特征
B组的小鼠模型<em>链球菌</em>阴道定植
从HIV-1感染细胞的外来体基于SILAC蛋白质组学表征
来自新世界Zika病毒感染性克隆的重组病毒的救援和表征
成年
日本血吸虫
细胞外囊泡的分离与鉴定
atp 利用商业检测和染色法
定量检测淋病奈瑟
菌骨料的抗生素敏感性
从人类唾液腺中分离唾液表皮细胞,作为脂质层或单层体进行体外生长
Read Article