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JoVE Journal
Immunology and Infection

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Metal-Begrænset vækst af Neisseria gonorrée for karakterisering af metal-responsive gener og metal erhvervelse fra Host Ligands
 

Metal-Begrænset vækst af Neisseria gonorrée for karakterisering af metal-responsive gener og metal erhvervelse fra Host Ligands

Article doi: 10.3791/60903
March 4th, 2020

Summary March 4th, 2020

Please note that all translations are automatically generated.

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Vi beskriver her en metode til vækst af Neisseria gonorrée i metal-begrænset flydende medium for at lette ekspressionen af gener for metaloptagelse. Vi skitserer også downstream eksperimenter for at karakterisere fænotype af gonococci dyrket under disse betingelser. Disse metoder kan tilpasses til at være egnet til karakterisering af metal-responsive gener i andre bakterier.

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