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Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response
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Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

DOI:
10.3791/60915-v

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06:26 min

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May 23, 2020

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, ,
1Department of Molecular Biology & Biochemistry,University of California, Irvine, 2Department of Physics & Astronomy,University of California, Irvine, 3Department of Veterinary and Animal Sciences,University of Copenhagen

Chapters

  • 00:04Introduction
  • 00:36Swarming Agar Plate Preparation
  • 01:32P. aeruginosa Growth and Plating
  • 03:30Time-Lapse Image Acquisition and Swarm Repulsion Measurement
  • 04:55Results: Representative Time-lapse Swarming and Collective Stress Response Imaging
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

We detail a simple method to produce high-resolution time-lapse movies of Pseudomonas aeruginosa swarms that respond to bacteriophage (phage) and antibiotic stress using a flatbed document scanner. This procedure is a fast and simple method for monitoring swarming dynamics and may be adapted to study the motility and growth of other bacterial species.

Tags

Keywords: Time-lapse ImagingBacterial SwarmingCollective Stress ResponsePseudomonas AeruginosaBacteriophageAntibioticsSwarming Agar PlatesDehumidifierDocument ScannerSwarming DynamicsPhage InfectionAntibiotic TreatmentConcentric Circle Plating

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