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En femtoliter droplet array for massivt parallell proteinsyntese fra enkelt DNA molekyler
JoVE Journal
Chemistry
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JoVE Journal Chemistry
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
DOI:

10:45 min

June 20, 2020

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Chapters

  • 00:04Introduction
  • 00:33Cleaning and Silanizing the Glass
  • 01:26Spin-Coating the Glass Substrate
  • 02:40Photolithography, Etching, and Cleaning
  • 03:47Fabricating the Microchamber Array Device
  • 05:04Generating the Femtoliter Droplet Array (FemDA)
  • 06:35Image Data Analysis
  • 08:45Results: Cell-Free Protein Synthesis in FemDA
  • 10:07Conclusion

Summary

Automatic Translation

Det overordnede målet med protokollen er å forberede over en million bestilte, ensartede, stabile og biokompatible femtoliterdråper på et 1 cm2 planar substrat som kan brukes til cellefri proteinsyntese.

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