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JoVE Journal
Developmental Biology

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Surgical Ablation Assay for Studying Eye Regeneration in Planarians
 

Surgical Ablation Assay for Studying Eye Regeneration in Planarians

Article DOI: 10.3791/55594
April 14th, 2017

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Summary April 14th, 2017

This protocol shows how to consistently excise planarian eyes (optic cups) without disturbing surrounding tissues. Using an insulin needle and syringe, either one or both eyes can be ablated to facilitate investigations into the mechanisms regulating eye regeneration, the evolution of visual regeneration, and the neural basis of light-induced behavior.

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