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Journal
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Biochemistry
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使用荧光寿命成像在老化
C.Elegans
中淀粉样蛋白结构的表征
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Characterization of Amyloid Structures in Aging
C. Elegans
Using Fluorescence Lifetime Imaging
Please note that all translations are automatically generated.
Click here for the English version.
使用荧光寿命成像在老化
C.Elegans
中淀粉样蛋白结构的表征
DOI:
10.3791/61004-v
•
09:31 min
•
March 27, 2020
•
Maria Lucia Pigazzini*
1,2
,
Christian Gallrein*
1
,
Manuel Iburg*
1
,
Gabriele Kaminski Schierle
,
Janine Kirstein
4
1
Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin
,
2
NeuroCure Cluster of Excellence
,
Charité – Universitätsmedizin Berlin
,
3
Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology
,
University of Cambridge
,
4
Cell Biology
,
University of Bremen
Chapters
00:05
Introduction
01:25
Synchronization of
C. elegans
and Mounting Nematodes onto Microscopy Slides
02:58
Acquisition of FLIM (Fluorescence Lifetime Imaging Microscopy) Data
04:32
Analysis of FLIM Data Using FLIMfit Software
07:15
Results: Fluorescence Lifetimes
08:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
荧光寿命成像监测器,定量和区分蛋白质在生活、衰老和压力
的C.elegans
疾病模型中的聚集趋势。
Tags
Amyloid Structures
Fluorescence Lifetime Imaging
Protein Aggregation
C. Elegans
Neurodegenerative Diseases
FLIM
Agarose Pads
Nematodes
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