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Biochemistry
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使用阿基-树脂辅助捕获检测蛋白质S-囊肿
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
Please note that all translations are automatically generated.
Click here for the English version.
使用阿基-树脂辅助捕获检测蛋白质S-囊肿
DOI:
10.3791/61016-v
•
08:31 min
•
April 10, 2020
•
Ritika Tewari*
1
,
Savannah J. West*
1,2
,
Bieerkehazi Shayahati
,
Askar M. Akimzhanov
1
Department of Biochemistry and Molecular Biology
,
McGovern Medical School at UT Health
,
2
MD Anderson UT Health Graduate School
Chapters
00:04
Introduction
01:25
Cell Lysate Preparation
02:26
Acyl-Resin Assisted Capture (Acyl-RAC): Free Thiol Group Blocking
03:54
Acyl-RAC: Hydroxylamine (HAM) Cleavage and S-Acylated Protein Capture
05:52
S-Acylated Protein Elution and Detection
06:52
Results: Representative S-Acylated Protein Detection
07:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
Acyl-RAC(阿西尔-树脂辅助捕获)是一种高度敏感、可靠且易于执行的方法,用于检测各种生物样品中半胱氨酸残留物(S-acycyy)的可逆脂改性。
Tags
Acyl-resin Assisted Capture
Acyl-RAC
Protein S-acylation
Thioester Bond Cleavage
Chloroform-methanol Precipitation
Protein Pellet
Cell Lysis
Protein Concentration Estimation
Bradford Assay
BCA Assay
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