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人体心室心肌细胞与颤音-切切心肌切片分离
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Biology
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JoVE Journal Biology
Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
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人体心室心肌细胞与颤音-切切心肌切片分离

DOI:
10.3791/61167-v

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11:19 min

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May 10, 2020

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, ,
1Institute of Cellular and Molecular Physiology,Friedrich-Alexander University Erlangen-Nürnberg, 2Muscle Research Center Erlangen (MURCE),Friedrich-Alexander-Universität Erlangen-Nürnberg

Chapters

  • 00:04Introduction
  • 00:41Preparing the Cardiac Tissue
  • 02:27Using the Vibratome
  • 04:00Tissue Digestion and Dissociation
  • 07:03Removing Mechanical Uncoupling Agent
  • 08:08Results: Vibratome-Cut Slices Yield Viable Cardiomyocytes
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

呈现是将人类和动物心室心肌细胞与振动切切心肌切片分离的协议。耐钙细胞（高达200个细胞/毫克）的高产量可以从少量组织（<50毫克）中获得。该协议适用于暴露于冷缺血的心肌，长达36小时。

Tags

Cardiomyocyte IsolationVibratomeMyocardial SlicesAgarose EmbeddingElectrophysiologyCardiac DiseaseHuman HeartAnimal Heart

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