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In Vivo Quantification du chiffre d’affaires des protéines dans le vieillissement C. Elegans à l’aide de Photoconvertible Dendra2
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In Vivo Quantification du chiffre d’affaires des protéines dans le vieillissement C. Elegans à l’aide de Photoconvertible Dendra2

Article DOI: 10.3791/61196-v • 09:45 min • June 13th, 2020
June 13th, 2020 •
1,2, 1,3
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Department of Biology and Chemistry, University of Bremen

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Présenté ici est un protocole pour surveiller la dégradation de la protéine huntingtine fusionnée au fluorophore photoconvertible Dendra2.

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Biologie Numéro 160 C. elegans huntingtine réseau de protéostase dégradation Dendra2 photoconversion microscopie confocale Fidji/ImageJ
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