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Journal
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Biochemistry
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使用亚细胞室特异性红氧敏感绿色荧光蛋白评估细胞氧化
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Biochemistry
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JoVE Journal
Biochemistry
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Please note that all translations are automatically generated.
Click here for the English version.
使用亚细胞室特异性红氧敏感绿色荧光蛋白评估细胞氧化
DOI:
10.3791/61229-v
•
06:10 min
•
June 18, 2020
•
Alev Tascioglu Aliyev
2
,
Francesca LoBianco
,
Kimberly J. Krager
,
Nukhet Aykin-Burns
1
Division of Radiation Health, Pharmaceutical Sciences
,
University of Arkansas for Medical Sciences
,
2
Department of Pharmaceutical Toxicology, Faculty of Pharmacy
,
Ege University
Chapters
00:04
Introduction
00:46
Cell Preparation
01:33
Adenoviral Redox Sensitive Green Fluorescent Protein (roGFP) Transduction
02:46
Cysteine/Cystine (CyS/CySS) Balance Acquisition
04:01
Results: Representative Cellular Oxidation Evaluation
05:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了对细胞内亚细胞室特异性氧化还原状态的评估。对氧化还原敏感的荧光探针可在完整细胞中方便地进行比例分析。
Tags
Subcellular Compartment
Redox-sensitive GFP
Oxidative Stress
Redox State
Ratiometric
Non-disruptive
Transduction
Flow Cytometry
Hydrogen Peroxide
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