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使用亚细胞室特异性红氧敏感绿色荧光蛋白评估细胞氧化
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
DOI:

06:10 min

June 18, 2020

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Chapters

  • 00:04Introduction
  • 00:46Cell Preparation
  • 01:33Adenoviral Redox Sensitive Green Fluorescent Protein (roGFP) Transduction
  • 02:46Cysteine/Cystine (CyS/CySS) Balance Acquisition
  • 04:01Results: Representative Cellular Oxidation Evaluation
  • 05:29Conclusion

Summary

Automatic Translation

该协议描述了对细胞内亚细胞室特异性氧化还原状态的评估。对氧化还原敏感的荧光探针可在完整细胞中方便地进行比例分析。

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