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Imagem e Análise do Transporte de Neurofilamento em Nervo Tibial de Camundongo Excised
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
DOI:

09:52 min

August 31, 2020

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Chapters

  • 00:04Introduction
  • 00:41Initial Assembly of Nerve Perfusion Chamber
  • 01:26Dissection and Preparation of Mouse Tibial Nerve
  • 03:09Final Nerve Perfusion Chamber Assembly
  • 04:48Fluorescence Activation and Image Acquisition
  • 06:21Flatfield and Darkfield Image Acquisition
  • 07:37Results: Pulse-escape and Pulse-spread Experiments
  • 09:17Conclusion

Summary

Automatic Translation

Descrevemos métodos de fotoativação de fluorescência para analisar o transporte axonal de neurofilamentos em axônios mieliados únicos de nervos periféricos de camundongos transgênicos que expressam uma proteína de neurofilamento fotoativavel.

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