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Developmental Biology
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解剖、免疫组织化学和幼虫和成年
果蝇
脑的安装用于视叶可视化
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Dissection, Immunohistochemistry and Mounting of Larval and Adult
Drosophila
Brains for Optic Lobe Visualization
Please note that all translations are automatically generated.
Click here for the English version.
解剖、免疫组织化学和幼虫和成年
果蝇
脑的安装用于视叶可视化
DOI:
10.3791/61273-v
•
11:29 min
•
April 28, 2021
•
Urfa Arain
,
Priscilla Valentino
,
Ishrat Maliha Islam
,
Ted Erclik
1
Departments of Biology and Cell & Systems Biology
,
University of Toronto – Mississauga
Chapters
00:00
Introduction
00:35
Larval Brain Dissection
02:23
Adult Brain Dissection
04:03
Mounting Larval Brains
06:06
Mounting Adult Brains
08:02
Representative Images of Larval and Adult Optic Lobes
10:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了准备幼虫和成年
果蝇
视叶进行成像的三个步骤:1)脑解剖,2)免疫组织化学和3)安装。重点放在第 3 步,因为需要不同的安装方向来可视化特定的光学瓣结构。
Tags
Drosophila
Brain Dissection
Optic Lobe
Immunohistochemistry
Mounting
Microscopy
Larva
Adult
Three-dimensional Visualization
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