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解剖、免疫组织化学和幼虫和成年 果蝇 脑的安装用于视叶可视化
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization
DOI:

11:29 min

April 28, 2021

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Chapters

  • 00:00Introduction
  • 00:35Larval Brain Dissection
  • 02:23Adult Brain Dissection
  • 04:03Mounting Larval Brains
  • 06:06Mounting Adult Brains
  • 08:02Representative Images of Larval and Adult Optic Lobes
  • 10:47Conclusion

Summary

Automatic Translation

该协议描述了准备幼虫和成年 果蝇 视叶进行成像的三个步骤:1)脑解剖,2)免疫组织化学和3)安装。重点放在第 3 步,因为需要不同的安装方向来可视化特定的光学瓣结构。

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